Potassium Chloride → Phosphate Buffered Saline

HRID 1680514Notes

Reaction equation

HRID 1680514 reaction equation
[Na+:1].[Cl-:2].[Cl-].[K+:4].[OH:5][P:6]([O-:9])([OH:8])=[O:7].[K+]>>[OH:7][P:6]([O-:9])([OH:8])=[O:5].[OH:7][P:6]([O-:9])([O-:8])=[O:5].[Na+:1].[Na+:1].[Na+:1].[Cl-:2].[Cl-:2].[K+:4].[K+:4]

Participants

4

Reactant 3

HCID 4873Potassium ChlorideHCID 5234Sodium ChlorideHCID 516951Monobasic potassium phosphate

Product 1

HCID 24978514Phosphate Buffered Saline

Procedure

The spent medium was poured off the monolayer and discarded. The monolayer then was rinsed twice with 10 ml phosphate buffered saline (PBS) with 0.02% ethylenediamine tetracetic acid (EDTA), at pH 7.4. The PBS was prepared by dissolving 80 grams NaCl, 2 grams KCl, 2 grams KH2PO4 and 21.6 grams Na2HPO4.7H2O in 10 liters distilled water.

Workup

  1. additionThe spent medium was poured off the monolayer
  2. washThe monolayer then was rinsed twice with 10 ml phosphate buffered saline (PBS) with 0.02% ethylenediamine tetracetic acid (EDTA), at pH 7.4
  3. distillationdistilled water

Sources & evidence

ORD record
ord-613c7ea8518c4170affc0f43b0f14e8d
Source dataset
uspto-grants-1981_06
Patent number
US04273871

Reaction notes

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