Sulfuric acid magnesium salt (1:1) → HCID 11027015, compound

Participants

Reaction Steps

Botryodioplodia sp. FI-741 was inoculated on 30 ml medium FI (2% potato starch, 2% ESUSAN Meat, 0.1% KH2PO4, 0.05% MgSO4 7H2O, 1% glucose, 0.05% Adekanol LG109) charged in an Erlenmeyer flask of 250 ml volume. Incubation was performed at 28° C. for 3 days. A solution of 60 mg of 1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)pyridine-3,5-dicarboxylic acid bis(2-nicotinoylaminoethyl)ester dihydrochloride in 0.75 ml of distilled water was added to the culture broth obtained. Shaking was continued for further 2 days at 28° C. Thereafter the culture broth was treated in a manner similar to Example 1-1) to give 8 mg of the title compound in an optical purity of 100%.

Provenance & Evidence