Sulfuric acid magnesium salt (1:1) → HCID 11027015, compound

HRID 1682417Notes

Reaction equation

HRID 1682417 reaction equation
OP([O-])(O)=O.[K+].[O-]S([O-])(=O)=O.[Mg+2].O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.Cl.Cl.[C:27]([NH:35][CH2:36][CH2:37][O:38][C:39]([C:41]1[CH:46]([C:47]2[CH:52]=[CH:51][CH:50]=[C:49]([N+:53]([O-:55])=[O:54])[CH:48]=2)[C:45]([C:56]([O:58]CCNC(=O)C2C=CC=NC=2)=[O:57])=[C:44]([CH3:70])[NH:43][C:42]=1[CH3:71])=[O:40])(=[O:34])[C:28]1[CH:33]=[CH:32][CH:31]=[N:30][CH:29]=1>O>[CH3:71][C:42]1[NH:43][C:44]([CH3:70])=[C:45]([C:56]([OH:58])=[O:57])[C@@H:46]([C:47]2[CH:52]=[CH:51][CH:50]=[C:49]([N+:53]([O-:55])=[O:54])[CH:48]=2)[C:41]=1[C:39]([O:38][CH2:37][CH2:36][NH:35][C:27](=[O:34])[C:28]1[CH:33]=[CH:32][CH:31]=[N:30][CH:29]=1)=[O:40]

Participants

6

Reactant 4

HCID 24083Sulfuric acid magnesium salt (1:1)HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 516951Monobasic potassium phosphateHCID 70183165HCID 70183165, compound

Product 1

HCID 11027015HCID 11027015, compoundYield 19.7%

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

Botryodioplodia sp. FI-741 was inoculated on 30 ml medium FI (2% potato starch, 2% ESUSAN Meat, 0.1% KH2PO4, 0.05% MgSO4 7H2O, 1% glucose, 0.05% Adekanol LG109) charged in an Erlenmeyer flask of 250 ml volume. Incubation was performed at 28° C. for 3 days. A solution of 60 mg of 1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)pyridine-3,5-dicarboxylic acid bis(2-nicotinoylaminoethyl)ester dihydrochloride in 0.75 ml of distilled water was added to the culture broth obtained. Shaking was continued for further 2 days at 28° C. Thereafter the culture broth was treated in a manner similar to Example 1-1) to give 8 mg of the title compound in an optical purity of 100%.

Workup

  1. additioncharged in an Erlenmeyer flask of 250 ml volume
  2. custombroth obtained
  3. waitwas continued for further 2 days at 28° C
  4. additionThereafter the culture broth was treated in a manner similar to Example 1-1)

Sources & evidence

ORD record
ord-90e0cbc468fe4002a5e4da7085e6b47e
Source dataset
uspto-grants-1997_06
Patent number
US05635395

Reaction notes

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