反应详情

To a mixture of (3R*,5S*)-3-amino-5-{cyclopropyl-[1-(3-methoxy-propyl)-1H-indol-3-yl-methyl]-carbamoyl}-piperidine-1-carboxylic acid 9H-fluoren-9-ylmethyl ester (121.4 mg, 0.2 mmol) (Starting Material 6), N-ethyldiisopropylamine (171.2 μL, 1 mmol) and 4-dimethylaminopyridine (10 mg) in CH2Cl2 (3 mL), neopentyl chloroformate (52.1 μL, 0.35 mmol) is added. After stirring at RT for 14 h, the mixture is diluted with CH2Cl2. The organic layer is washed with 1N HCl and saturated NaHCO3 solution, dried over Na2SO4 and evaporated in vacuo. The crude product is stirred with a solution of CH2Cl2/piperidine 4:1 (5 mL) for 2 h, the mixture is evaporated and the residue purified by preparative HPLC (C18 column, 10-100% CH3CN+0.1% TFA/H2O+0.1% TFA, 20 min, flow 20 mL/min). The combined pure fractions are treated with K2CO3, CH3CN is removed in vacuo, and the residual aqueous phase is extracted three times with CH2Cl2. The combined organic layers are dried (Na2SO4) and evaporated to afford the title compound as a yellowish solid. MS: 499.2 [M+H]+; tR (HPLC, Nucleosil C18; 5-100% CH3CN+0.1% TFA/H2O+0.1% TFA for 8 min, flow 1.5 ml/min): 5.45 min.

1. additionis added
2. washThe organic layer is washed with 1N HCl and saturated NaHCO3 solution
3. dry with materialdried over Na2SO4
4. customevaporated in vacuo
5. stirringThe crude product is stirred with a solution of CH2Cl2/piperidine 4:1 (5 mL) for 2 h
6. customthe mixture is evaporated
7. customthe residue purified by preparative HPLC (C18 column, 10-100% CH3CN+0.1% TFA/H2O+0.1% TFA, 20 min, flow 20 mL/min)
8. additionThe combined pure fractions are treated with K2CO3, CH3CN
9. customis removed in vacuo
10. extractionthe residual aqueous phase is extracted three times with CH2Cl2
11. dry with materialThe combined organic layers are dried (Na2SO4)
12. customevaporated