Dimethylformamide → Glucose Monohydrate

Participants

Reaction Steps

Between about 36 and 48 hours of fermentation time, steffimycin B is added to the fermentation as a dimethylformamide solution (25 mg/ml) to a final medium concentration of 25 mg/l. The fermentation is monitored using tlc on silica gel plates with CHCl3 :CH3OH (925:75) as the developing solvent system. The tlc procedure is conducted at half day intervals between the first half day and five days.

Provenance & Evidence