Ammonia → HCID 101281155, compound

HRID 1707032Notes

Reaction equation

HRID 1707032 reaction equation
C(Cl)(Cl)Cl.C(O)C.[NH3:8].[CH3:9][C@H:10]([NH2:35])[CH:11]1[O:16][C@@H:15]([O:17][C@H:18]2[C@H:23]([OH:24])[C@H:22]([N:25](C(CN)=O)[CH3:26])[C@@H:21]([O:31][CH3:32])[C@@H:20]([OH:33])[C@@H:19]2[NH2:34])[CH2:14][CH2:13][CH2:12]1>CC(C)=O>[CH3:9][C@H:10]([NH2:35])[C:11]1[O:16][C@H:15]([O:17][C@H:18]2[C@H:23]([OH:24])[C@H:22]([NH:25][CH3:26])[C@@H:21]([O:31][CH3:32])[C@@H:20]([OH:33])[C@@H:19]2[NH2:34])[C@H:14]([NH2:8])[CH2:13][CH:12]=1

Participants

6

Reactant 4

HCID 222AmmoniaHCID 702EthanolHCID 6212ChloroformHCID 52946017HCID 52946017, compound

Product 1

HCID 101281155HCID 101281155, compound

Reagents, catalysts & solvents 1

HCID 180AcetoneSolvent

Procedure

The crude powders are then subjected to silica gel column chromatography using a mixed solvent of chloroform, ethanol, aqueous ammonia and acetone (2:2:1:2, by volume) as a developer. The crude powder containing Fortimicin factors B, KE and KG is suspended in the solvent and introduced into the column. Development is carried out with the same solvent at a flow rate of about 30 ml/hour. First, Fortimicin B and Fortimicin KE are eluted and then Fortimicin KG is eluted. The active fractions containing Fortimicin KG are collected and concentrated under reduced pressure. The concentrate is freeze-dried to obtain the free base of Fortimicin KG.

Workup

  1. additionintroduced into the column
  2. washFirst, Fortimicin B and Fortimicin KE are eluted
  3. washFortimicin KG is eluted
  4. additionThe active fractions containing Fortimicin KG
  5. customare collected
  6. concentrationconcentrated under reduced pressure
  7. customThe concentrate is freeze-dried

Sources & evidence

ORD record
ord-8247fea6c93a45039404812714616fde
Source dataset
uspto-grants-1980_06
Patent number
US04209612

Reaction notes

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