Ammonia → HCID 101281155, compound

Participants

Reaction Steps

The crude powders are then subjected to silica gel column chromatography using a mixed solvent of chloroform, ethanol, aqueous ammonia and acetone (2:2:1:2, by volume) as a developer. The crude powder containing Fortimicin factors B, KE and KG is suspended in the solvent and introduced into the column. Development is carried out with the same solvent at a flow rate of about 30 ml/hour. First, Fortimicin B and Fortimicin KE are eluted and then Fortimicin KG is eluted. The active fractions containing Fortimicin KG are collected and concentrated under reduced pressure. The concentrate is freeze-dried to obtain the free base of Fortimicin KG.

Provenance & Evidence