Phosphate → 7-Aminocephalosporanic acid

Participants

Reaction Steps

1 g of the cellular paste so obtained was suspended in 50 ml. of phosphate buffer 0,1M, pH 8,0, containing 2% glutaryl-7ACA, and incubated for 10 min. at 37° C. After such period of time, the reaction was stopped by the addition of 20% acetic acid, at the rate of 3 ml for 1 ml of the reaction mixture. The suspension obtained was centrifuged and the 7-ACA formed was quantified in the clear supernatant.

Provenance & Evidence