Phosphate → 7-Aminocephalosporanic acid

HRID 1707556Notes

Reaction equation

HRID 1707556 reaction equation
P([O-])([O-])([O-])=O.[CH3:6][C:7]([O:9][CH2:10][C:11]1[CH2:28][S:27][C@@H:14]2[C@H:15]([NH:18]C(CCCC(O)=O)=O)[C:16](=[O:17])[N:13]2[C:12]=1[C:29]([OH:31])=[O:30])=[O:8]>C(O)(=O)C>[CH3:6][C:7]([O:9][CH2:10][C:11]1[CH2:28][S:27][C@@H:14]2[C@H:15]([NH2:18])[C:16](=[O:17])[N:13]2[C:12]=1[C:29]([OH:31])=[O:30])=[O:8]

Participants

4

Reactant 2

HCID 1061PhosphateHCID 446570Glutaryl-7-aminocephalosporanic acid

Product 1

HCID 4413287-Aminocephalosporanic acid

Reagents, catalysts & solvents 1

HCID 176Acetic AcidSolvent

Procedure

1 g of the cellular paste so obtained was suspended in 50 ml. of phosphate buffer 0,1M, pH 8,0, containing 2% glutaryl-7ACA, and incubated for 10 min. at 37° C. After such period of time, the reaction was stopped by the addition of 20% acetic acid, at the rate of 3 ml for 1 ml of the reaction mixture. The suspension obtained was centrifuged and the 7-ACA formed was quantified in the clear supernatant.

Workup

  1. custom1 g of the cellular paste so obtained
  2. waitAfter such period of time
  3. customThe suspension obtained

Sources & evidence

ORD record
ord-ced8de0378d44961982db6546dedd50b
Source dataset
uspto-grants-1994_10
Patent number
US05354667

Reaction notes

Loading…