反应详情

1.06 g (6mmol) of 5-methyl-4-(1,2,4-triazol-1-yl)-pyrimidin-2(1H)-one are dissolved in 20 ml of 40% strength aqueous methylamine solution and the solution is stirred for 5 hours at room temperature. The solution is concentrated and chromatographed on silica gel using ethyl acetate/methanol 2/1. 0.67 g (80.3% of theory) of N4-methyl-5-methylcytosine is obtained as a colorless powder with melting point of 160°-165° C. (decomp.). 1H NMR, d6 -DMSO, δ[ppm]: 10.4 (s, 1H) 7.17 (s, 1H), 7.1 (s, broad, 1H), 2.8 (d, 3H), 1.82 (d, 3H).

1. concentrationThe solution is concentrated
2. customchromatographed on silica gel