HuagongsheHuagongshe Chemistry Print Report · For research reference only
Chloroform → N-(1-(2,3-dioleyloxy)propyl)-N,N,N-trimethylammonium
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Chloroform | HCID 6212 | — |
| Reactant | Maleimide | HCID 10935 | — |
| Reactant | 1,2-Dioleoyl-sn-glycero-3-phospho(1-rac-glycerol) | HCID 119120 | — |
| Reactant | 1,2-Dioleoyl-sn-glycero-3-phosphocholine | HCID 10350317 | — |
| Reactant | Dopg dopc | HCID 131716291 | — |
| Product | N-(1-(2,3-dioleyloxy)propyl)-N,N,N-trimethylammonium | HCID 6438350 | — |
Reaction Steps
DOPG/DOPC sonicated vesicles containing maleimide-PE were prepared by dissolving 16 mg DOPG, 24 mg DOPC and 0.4 mg maleimide-PE in chloroform and removing the solvent by drying under a stream of nitrogen gas. This dried film was placed under vacuum overnight to remove traces of solvent. The film was then suspended in 0.8 ml water with vigorous shaking and sonicated in a bath type sonicator for 30 minutes until clear producing DOTMA/DOPPC SUV's at a concentration of 50 mg/ml.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US04946787 |
| Source dataset | uspto-grants-1990_08 |
| ORD ID | ord-c3ec95f0568040cdacff9f366921d38a |
| Source note | uspto-grants-1990_08 |