反应详情

Pyrazolo[1,5-a]pyridine-3-carbonylchloride (100 mg, 0.55 mmol, Maybridge Lot # 291259) and N-(3-amino-4-methyl-phenyl)-3-trifluoromethyl-benzamide (163 mg, 0.55 mmol) are dissolved in 2 ml dry methylenehloride. Triethylamine (93 μl, 0.66 mmol) is added and the reaction mixture is stirred at room temperature. After complete formation of the product, the reaction mixture is quenched with water and the product is extracted with methylenehloride. The solvent is removed under reduced pressure and the crude product is absorbed to Isolute. The product is purified by automated reversed phase column chromatography (column: Interchrom Puriflash 15/35U C18, 70 g cartouche; solvents: water+0.1% trifluoroacetic acid and acetonitrile+0.1% trifluoroacetic acid) and is dried at the high vacuum pump, yielding the title compound as a white solid.

1. customdry methylenehloride
2. customAfter complete formation of the product, the reaction mixture is quenched with water
3. extractionthe product is extracted with methylenehloride
4. customThe solvent is removed under reduced pressure
5. customthe crude product is absorbed to Isolute
6. customThe product is purified by automated reversed phase column chromatography (column: Interchrom Puriflash 15/35U C18, 70 g cartouche; solvents: water+0.1% trifluoroacetic acid and acetonitrile+0.1% trifluoroacetic acid)
7. customis dried at the high vacuum pump