Mops → Carvone, (+-)-

HRID 1716176Notes

Reaction equation

[CH3:1][C:2]1[CH2:7][CH2:6][C@@H:5]([C:8]([CH3:10])=[CH2:9])[CH2:4][CH:3]=1.C1N(CCCS(O)(=O)=O)CC[O:13]C1>>[CH3:1][C:2]1[C:7](=[O:13])[CH2:6][CH:5]([C:8]([CH3:10])=[CH2:9])[CH2:4][CH:3]=1

Participants

Reactant 2

HCID 70807Mops

HCID 440917(+)-Limonene

Product 1

HCID 7439Carvone, (+-)-

Procedure

For transformation of limonene 50 mg Pleurotus sapidus mycel was placed in 1.5 mL MOPS buffer (0.1 M; pH 7.0) and the dried cell mass was rehydrated for one hour at 200 rpm and 24° C. For producing carvone 41 mM limonene were directly applied to the rehydrated culture. The reaction took place for 24 h at 150 rpm and 24° C. After addition of the internal standard (for example camphor for limonene transformation) samples were extracted with 2 mL azeotropic pentane/ether mixture, centrifuged and dried over night with Na2SO4.

Sources & evidence

ORD record: ord-3726e9ac99af47cebd5253e1982adb3c
Source dataset: uspto-grants-2010_09
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US07803606B2

Reaction notes

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