Hydrochloric Acid → Tryptophan--hydrogen chloride (1/1)

Participants

Reaction Conditions

Reaction Steps

The pH of 30 l of L-tryptophan fermentation broth was adjusted to 3.5 with 35% hydrochloric acid. After the cells were removed from the broth by centrifugation, the solution was concentrated at 60° C. under reduced pressure until the concentration of L-tryptophan reached 180 g/l. Next, 5 equivalents of 35% hydrochloric acid were added to the crystallization slurry (relative to the amount of L-tryptophan), while the slurry was maintained at a temperature of 50° C. After cooling to 15° C. overnight, the crystals were separated with a centrifuging machine, and washed with water. The crystals were dried at 60° C. for 2 hours under reduced pressure to give 161 g of L-tryptophan hydrochloride crystals. The L-tryptophan hydrochloride crystals had a purity of 95.7%.

Provenance & Evidence