Hydrochloric Acid → Tryptophan--hydrogen chloride (1/1)

HRID 1728912Notes

Reaction equation

HRID 1728912 reaction equation
[NH2:1][C@H:2]([C:13]([OH:15])=[O:14])[CH2:3][C:4]1[C:12]2[C:7](=[CH:8][CH:9]=[CH:10][CH:11]=2)[NH:6][CH:5]=1.[ClH:16]>>[ClH:16].[NH2:1][C@H:2]([C:13]([OH:15])=[O:14])[CH2:3][C:4]1[C:12]2[C:7](=[CH:8][CH:9]=[CH:10][CH:11]=2)[NH:6][CH:5]=1

Participants

3

Reactant 2

HCID 313Hydrochloric Acid2×HCID 6305L-Tryptophan2×

Product 1

HCID 134608Tryptophan--hydrogen chloride (1/1)

Conditions

Temperature
50 °C

Procedure

The pH of 30 l of L-tryptophan fermentation broth was adjusted to 3.5 with 35% hydrochloric acid. After the cells were removed from the broth by centrifugation, the solution was concentrated at 60° C. under reduced pressure until the concentration of L-tryptophan reached 180 g/l. Next, 5 equivalents of 35% hydrochloric acid were added to the crystallization slurry (relative to the amount of L-tryptophan), while the slurry was maintained at a temperature of 50° C. After cooling to 15° C. overnight, the crystals were separated with a centrifuging machine, and washed with water. The crystals were dried at 60° C. for 2 hours under reduced pressure to give 161 g of L-tryptophan hydrochloride crystals. The L-tryptophan hydrochloride crystals had a purity of 95.7%.

Workup

  1. customAfter the cells were removed from the broth by centrifugation
  2. concentrationthe solution was concentrated at 60° C. under reduced pressure until the concentration of L-tryptophan
  3. temperatureAfter cooling to 15° C. overnight
  4. customthe crystals were separated with a centrifuging machine
  5. washwashed with water
  6. customThe crystals were dried at 60° C. for 2 hours under reduced pressure

Sources & evidence

ORD record
ord-8f5567007b5a4b8a89f3299c1b1c6b03
Source dataset
uspto-grants-1994_07
Patent number
US05329014

Reaction notes

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