Hydrochloric Acid → Tryptophan--hydrogen chloride (1/1)

Participants

Reaction Conditions

Reaction Steps

The pH of an L-tryptophan fermentation broth was adjusted to 3.5 with 35% hydrochloric acid. After the cells were removed from the broth by centrifugation, the solution was concentrated at a temperature of 60° C. under reduced pressure until the concentration of L-tryptophan reached 150 g/l. After cooling the concentrated slurry to 15° C. overnight, the crystals were separated with a centrifuging machine, and were washed with water. The crystals were dried at 60° C. for 2 hours under reduced pressure to give 106 g of L-tryptophan hydrochloride crystals. The L-tryptophan hydrochloride crystals were obtained in a yield of 60%, and had a purity of 71.8%.

Provenance & Evidence