Hydrochloric Acid → Tryptophan--hydrogen chloride (1/1)

HRID 1728913Notes

Reaction equation

HRID 1728913 reaction equation
[NH2:1][C@H:2]([C:13]([OH:15])=[O:14])[CH2:3][C:4]1[C:12]2[C:7](=[CH:8][CH:9]=[CH:10][CH:11]=2)[NH:6][CH:5]=1.[ClH:16]>>[ClH:16].[NH2:1][C@H:2]([C:13]([OH:15])=[O:14])[CH2:3][C:4]1[C:12]2[C:7](=[CH:8][CH:9]=[CH:10][CH:11]=2)[NH:6][CH:5]=1

Participants

3

Reactant 2

HCID 313Hydrochloric AcidHCID 6305L-Tryptophan

Product 1

HCID 134608Tryptophan--hydrogen chloride (1/1)Yield 60%

Conditions

Temperature
15 °C

Procedure

The pH of an L-tryptophan fermentation broth was adjusted to 3.5 with 35% hydrochloric acid. After the cells were removed from the broth by centrifugation, the solution was concentrated at a temperature of 60° C. under reduced pressure until the concentration of L-tryptophan reached 150 g/l. After cooling the concentrated slurry to 15° C. overnight, the crystals were separated with a centrifuging machine, and were washed with water. The crystals were dried at 60° C. for 2 hours under reduced pressure to give 106 g of L-tryptophan hydrochloride crystals. The L-tryptophan hydrochloride crystals were obtained in a yield of 60%, and had a purity of 71.8%.

Workup

  1. customAfter the cells were removed from the broth by centrifugation
  2. concentrationthe solution was concentrated at a temperature of 60° C. under reduced pressure until the concentration of L-tryptophan
  3. customthe crystals were separated with a centrifuging machine
  4. washwere washed with water
  5. customThe crystals were dried at 60° C. for 2 hours under reduced pressure

Sources & evidence

ORD record
ord-1028abcdd91744eab37f339c7e0b0db9
Source dataset
uspto-grants-1994_07
Patent number
US05329014

Reaction notes

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