Phosphate → Phosphate

Participants

Reaction Steps

A DL-Tryptophane methyl ester (10 mM) in 0.02 M phosphate buffer, pH 7.5, was pressed through a column of chymotrypsinsephadex particles. The permeate contained a mixture of L-tryptophane (5 mM) obtained by stereo-specific enzymatic hydrolysis, and D-tryptophane methyl ester (5 mM) and 20 mM phosphate buffer, pH 7.0. This mixture was separated in an electrodialysis cell as described in Example 1. Electric current of 5 mA/cm2 was applied through the electrodialysis cell in which the feed solution was circulated with a linear flow velocity of about 5 cm/sec. At the end of the experiment, the acid in the feed compartment was tested for chemical and optical purity. It was found to be 98.5% pure L-tryptophane.

Provenance & Evidence