Phosphate → HCID 20485290, compound

HRID 1732671Notes

Reaction equation

HRID 1732671 reaction equation
[CH2:1]([C:4]1([CH2:12][CH2:13][CH3:14])[CH2:10][CH2:9][C:8](=[O:11])[O:7][C:5]1=[O:6])[CH2:2][CH3:3].P([O-])([O-])([O-])=[O:16]>C1COCC1>[CH2:1]([C:4]([CH2:10][CH2:9][C:8]([OH:7])=[O:11])([CH2:12][CH2:13][CH3:14])[C:5]([OH:16])=[O:6])[CH2:2][CH3:3]

Participants

4

Reactant 2

HCID 1061PhosphateHCID 53658587HCID 53658587, compound

Product 1

HCID 20485290HCID 20485290, compound

Reagents, catalysts & solvents 1

HCID 8028TetrahydrofuranSolvent

Procedure

To a solution of BSA (300 mg, 5×10-4 mmole) in 20 ml of phosphate buffer was added the hapten, 2,2-dipropylglutaric anhydride as prepared in Example 5 (55 mg, 0.25 mmole in 200 ml THF) in 4μl portions over an hour. The resulting clear solution was stirred at 4° overnight, after which it was dialyzed in 7×2 liter of water adjusted to pH 8 with phosphate buffer, followed by one time with deionized water. The dialysis took 5 days. Lyophilization gave 320 mg of conjugate having a hapten number of 40 as estimated by the amino group precipitation method.

Workup

  1. customafter which it was dialyzed in 7×2 liter of water
  2. customThe dialysis
  3. waittook 5 days
  4. customLyophilization gave 320 mg of conjugate
  5. customas estimated by the amino group precipitation method

Sources & evidence

ORD record
ord-7eb5b114a43a4b66be6c4812fe1c8809
Source dataset
uspto-grants-1980_12
Patent number
US04238389

Reaction notes

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