Isobutyric Acid → Acetate

HRID 1735665Notes

Reaction equation

HRID 1735665 reaction equation
[C:1]([O-:6])(=[O:5])[C@H:2]([CH3:4])[OH:3].[Na+].[C:8]([OH:13])(=[O:12])[CH:9](C)C.[OH-].[Na+].CCCCCCCCCCCCC[N+](CC1C=CC=CC=1)(C)C.[Cl-]>>[C:1]([O-:6])(=[O:5])[C:2]([CH3:4])=[O:3].[C:8]([O-:13])(=[O:12])[CH3:9]

Participants

6

Reactant 4

HCID 6590Isobutyric AcidHCID 14798Sodium HydroxideHCID 3014024Benzalkonium ChlorideHCID 23664767Sodium Lactate, L-

Product 2

HCID 175AcetateHCID 107735Pyruvate

Procedure

The procedure described in Example 1 was repeated using 10 mL of an aqueous solution containing sodium L-lactate (0.500M) and isobutyric acid (HPLC internal standard, 0.100M) at pH 9.0 (adjusted with 50% NaOH), to which was added 0.35 g (wet weight) of Pichia pastoris transformant GS115-MSP10 (2.26 IU/mL glycolate oxidase and 5,000 IU/mL catalase) which had been permeabilized by treatment with 0.1% benzalkonium chloride ("BARQUAT" OJ-50); no buffer was added. After 8 hours, the HPLC yields of pyruvate and acetate were 97.4% and 2.3%, respectively, and 0.4% lactate remained. The remaining permeabilized cell activity of glycolate oxidase and catalase were 123% and 150%, respectively, of their initial values.

Workup

  1. additionno buffer was added

Sources & evidence

ORD record
ord-85fa0e14f2d24b659d248a1500b28805
Source dataset
uspto-grants-1996_07
Patent number
US05538875

Reaction notes

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