Isobutyric Acid → Acetate

Participants

Reaction Steps

The procedure described in Example 1 was repeated using 10 mL of an aqueous solution containing sodium L-lactate (0.500M) and isobutyric acid (HPLC internal standard, 0.100M) at pH 9.0 (adjusted with 50% NaOH), to which was added 0.35 g (wet weight) of Pichia pastoris transformant GS115-MSP10 (2.26 IU/mL glycolate oxidase and 5,000 IU/mL catalase) which had been permeabilized by treatment with 0.1% benzalkonium chloride ("BARQUAT" OJ-50); no buffer was added. After 8 hours, the HPLC yields of pyruvate and acetate were 97.4% and 2.3%, respectively, and 0.4% lactate remained. The remaining permeabilized cell activity of glycolate oxidase and catalase were 123% and 150%, respectively, of their initial values.

Provenance & Evidence