Oxygen → Acetate

HRID 1735668Notes

Reaction equation

HRID 1735668 reaction equation
[C:1]([O-:6])(=[O:5])[C@H:2]([CH3:4])[OH:3].[Na+].[C:8]([OH:13])(=[O:12])[CH:9](C)C.[OH-].[Na+].C([O-])(=O)CO.CCCCCCCCCCCCC[N+](CC1C=CC=CC=1)(C)C.[Cl-].O=O>>[C:1]([O-:6])(=[O:5])[C:2]([CH3:4])=[O:3].[C:8]([O-:13])(=[O:12])[CH3:9]

Participants

8

Reactant 6

HCID 977OxygenHCID 6590Isobutyric AcidHCID 14798Sodium HydroxideHCID 3014024Benzalkonium ChlorideHCID 5460308HydroxyacetateHCID 23664767Sodium Lactate, L-

Product 2

HCID 175AcetateHCID 107735Pyruvate

Procedure

The procedure described in Example 1 was repeated using 10 mL of an aqueous solution containing sodium L-lactate (0.50M) and isobutyric acid (HPLC internal standard, 0.100M) at pH 9.0 (adjusted with 50% NaOH), to which was added 0.66 g (wet weight) of Pichia pastoris transformant GS115-MSP10 (6.27 IU/mL glycolate oxidase and I0,000 IU/mL catalase) which had been permeabilized by treatment with 0.1% benzalkonium chloride ("BARQUAT" OJ-50); no buffer was added. The reaction temperature was 15° C. and the oxygen pressure was 70 psig. After 3 hours, the HPLC yields of pyruvate and acetate were 98.2% and 1.2%, respectively, and 0.6% lactate remained. The remaining permeabilized cell activity of glycolate oxidase and catalase were 124% and 130%, respectively, of their initial values.

Workup

  1. additionno buffer was added
  2. customwas 15° C.

Sources & evidence

ORD record
ord-204cab01e9054049ae98aca42f888958
Source dataset
uspto-grants-1996_07
Patent number
US05538875

Reaction notes

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