Oxygen → Acetate

Participants

Reaction Steps

The procedure described in Example 1 was repeated using 10 mL of an aqueous solution containing sodium L-lactate (0.50M) and isobutyric acid (HPLC internal standard, 0.100M) at pH 9.0 (adjusted with 50% NaOH), to which was added 0.66 g (wet weight) of Pichia pastoris transformant GS115-MSP10 (6.27 IU/mL glycolate oxidase and I0,000 IU/mL catalase) which had been permeabilized by treatment with 0.1% benzalkonium chloride ("BARQUAT" OJ-50); no buffer was added. The reaction temperature was 15° C. and the oxygen pressure was 70 psig. After 3 hours, the HPLC yields of pyruvate and acetate were 98.2% and 1.2%, respectively, and 0.6% lactate remained. The remaining permeabilized cell activity of glycolate oxidase and catalase were 124% and 130%, respectively, of their initial values.

Provenance & Evidence