Oxygen → Acetate

HRID 1735673Notes

Reaction equation

HRID 1735673 reaction equation
[C:1]([O-:6])(=[O:5])[C@H:2]([CH3:4])[OH:3].[Na+].[OH-].[Na+].[C:10]([O-:14])(=[O:13])[CH2:11]O.CCCCCCCCCCCCC[N+](CC1C=CC=CC=1)(C)C.[Cl-].O=O>>[C:1]([O-:6])(=[O:5])[C:2]([CH3:4])=[O:3].[C:10]([O-:14])(=[O:13])[CH3:11]

Participants

7

Reactant 5

HCID 977OxygenHCID 14798Sodium HydroxideHCID 3014024Benzalkonium ChlorideHCID 5460308HydroxyacetateHCID 23664767Sodium Lactate, L-

Product 2

HCID 175AcetateHCID 107735Pyruvate

Procedure

The procedure described in Example 11 was repeated using 100 mL of an aqueous solution containing sodium L-lactate (0.532M) at pH 7.4 (adjusted with 50% NaOH), to which was added only 1.04 g (wet weight) of Pichia pastoris transformant GS115-MSP 10 (0.52 IU/mL glycolate oxidase and 2,230 IU/mL catalase) which had been permeabilized by treatment with 0.1% benzalkonium chloride ("BARQUAT" MB-50); no buffer was added. The reaction temperature was 5° C. and the oxygen pressure was 70 psig. After 24 hours, the HPLC yields of pyruvate and acetate were 93.7% and 5.0%, respectively, and 2.8% lactate remained. The remaining permeabilized cell activity of glycolate oxidase and catalase were 79% and 43%, respectively, of their initial values.

Workup

  1. additionno buffer was added
  2. customwas 5° C.

Sources & evidence

ORD record
ord-d4a2c3179feb4a71b35405a85c964d47
Source dataset
uspto-grants-1996_07
Patent number
US05538875

Reaction notes

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