Oxygen → Acetate

Participants

Reaction Steps

The procedure described in Example 11 was repeated using 100 mL of an aqueous solution containing sodium L-lactate (0.532M) at pH 7.4 (adjusted with 50% NaOH), to which was added only 1.04 g (wet weight) of Pichia pastoris transformant GS115-MSP 10 (0.52 IU/mL glycolate oxidase and 2,230 IU/mL catalase) which had been permeabilized by treatment with 0.1% benzalkonium chloride ("BARQUAT" MB-50); no buffer was added. The reaction temperature was 5° C. and the oxygen pressure was 70 psig. After 24 hours, the HPLC yields of pyruvate and acetate were 93.7% and 5.0%, respectively, and 2.8% lactate remained. The remaining permeabilized cell activity of glycolate oxidase and catalase were 79% and 43%, respectively, of their initial values.

Provenance & Evidence