Acetate → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

The immobilized enzyme conjugate was assayed at a temperature of 55° C. using as substrate 50 ml of 30% (w/v) corn starch in 0.02M acetate at a pH of 4.2. To carry out the assay, the substrate and enzyme were placed in 250 ml flask and incubated in a 55° C. shaker water bath. At 15 and 75 minutes of reaction time, a 0.2 ml aliquot of reaction mixture was removed and added to 0.5 ml of 0.2N H2SO4 to terminate the reaction. These samples were then diluted by addition of 5.3 ml of distilled water, and then filtered through 0.45μ filter. The products were analyzed by carbohydrate high pressure liquid chromatography (HPLC). One unit of activity represents the amount of enzyme which will produce one micromole of glucose in one minute under the conditions of the assay, and is reported as U/g (units of activity per gram of conjugate).

1. customwas assayed at a temperature of 55° C.
2. customthe substrate and enzyme were placed in 250 ml flask
3. customincubated in a 55° C.
4. customAt 15 and 75 minutes of reaction time
5. customa 0.2 ml aliquot of reaction mixture
6. customwas removed
7. additionadded to 0.5 ml of 0.2N H2SO4
8. customto terminate
9. customthe reaction
10. filtrationfiltered through 0.45μ
11. filtrationfilter

ORD record

ord-bc4a32b4e91a40cd865705180cd3c3cf

Source dataset

uspto-grants-1996_07

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US05541097