L-(-)-Sorbose → 2-keto-L-Gulonic acid

Gluconobacter oxydans L-8 (FERM-P No. 8355) was cultivated in a 30 l jar fermentor in the same manner as described in Example 5. After 38 hours of cultivation, 1.5 l of the culture was harvested to obtain the cells. 10 g of the cells were suspended into 60 ml of 10 mM potassium phosphate buffer (pH, 7.0) and homogenized by a Dyno mill homogenizer (Willy, A. Bachofen Co.) with glass beads at 2000 rpm for 4 minutes at 4° C. The homogenate was centrifuged at 5000 rpm for 10 minutes to remove the intact cells. 2 ml of the supernatant thus obtained was mixed with 1 ml of 0.5M potassium phosphate buffer (pH, 7.0) and 6 ml of water containing 100 mg of L-sorbose and incubated at 30° C. for 4 hours. As a result, 25 mg of 2-keto-L-gulonic acid was formed.

1. customto obtain the cells
2. wait) with glass beads at 2000 rpm for 4 minutes at 4° C
3. waitThe homogenate was centrifuged at 5000 rpm for 10 minutes
4. customto remove the intact cells
5. custom2 ml of the supernatant thus obtained
6. waitincubated at 30° C. for 4 hours

ORD record

ord-657de811601d4f17b585fd8693172abd

Source dataset

uspto-grants-1996_07

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US05541108