反应详情

The compound 14 (6.9 mg) was dissolved in methanol (0.4 mL), a 2M aqueous sodium hydroxide solution (0.1 mL) was added at room temperature, and the mixture was stirred overnight. Dilute hydrochloric acid was added to the reaction liquid, and the mixture was then extracted with ethyl acetate two times. The organic layers were combined, washed with a saturated saline, and dried with anhydrous magnesium sulfate. The residue obtained by concentration under reduced pressure was purified by silica gel column chromatography (dichloromethane: methanol=100:1→10:1) to obtain a titled compound (4.8 mg) having the following physical property values.

1. extractionthe mixture was then extracted with ethyl acetate two times
2. washwashed with a saturated saline
3. dry with materialdried with anhydrous magnesium sulfate
4. customThe residue obtained by concentration under reduced pressure
5. customwas purified by silica gel column chromatography (dichloromethane: methanol=100:1→10:1)