Glutathione → HCID 178514376, compound

HRID 1748712Notes

Reaction equation

HRID 1748712 reaction equation
[CH:1]1[N:5]=[CH:4][NH:3][C:2]=1/[CH:6]=[CH:7]/[C:8]([OH:10])=[O:9].[O-]P(OP([O-])([O-])=O)(=O)[O-].[NH2:20][C@@H](CCC(N[C@H](C(NCC(O)=O)=O)CS)=O)C(O)=O>[Cl-].[Cl-].[Zn+2].N[C@H](C(O)=O)CC1N=CNC=1>[NH3:3].[NH2:20][C@H:7]([C:8]([OH:10])=[O:9])[CH2:6][C:2]1[N:3]=[CH:4][NH:5][CH:1]=1

Participants

6

Reactant 3

HCID 124886GlutathioneHCID 644102PyrophosphateHCID 736715trans-Urocanic acid

Product 1

HCID 178514376HCID 178514376, compound

Reagents, catalysts & solvents 2

HCID 3007855ZincchlorideCatalystHCID 178419078HCID 178419078, compoundSolvent

Procedure

HAL enzyme activity can be determined as described in Baedeker & Schulz (Eur. J. Biochem 2002, 269, 1790-1797), wherein enzyme activity was determined as the rate of urocanate formation, measured spectrophotometrically at 277 nm. For a standard assay, the enzyme was preincubated at 25° C. for 5 min in 2.5 mL buffer containing 0.1 M pyrophosphate (pH 9.3), 10 μM ZnCl2 and 2 mM glutathione. The reaction was started by adding 200 μL of 0.5 M histidine solution and then monitored for approximately 5 minutes.

Workup

  1. waitmonitored for approximately 5 minutes

Sources & evidence

ORD record
ord-55c83ba394024cfdb19a1a24bbb667c8
Source dataset
uspto-grants-2015_10
Patent number
US09150845B2

Reaction notes

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