反应详情

Clean glassware to be used with 2 M NaOH for 3-5 minutes and the rinse thoroughly with deionized water. Prepare a first saline buffer by adding 2.7017 grams of dibasic sodium phosphate, USP to 5 liters of 0.9% sodium chloride for injection, USP. Prepare a second saline buffer by adding 1.39 grams of monobasic sodium phosphate, USP to 1 liter of 0.9% sodium chloride for injection, USP. While stirring the first saline buffer solution, slowly add the second saline buffer solution until the final pH is 7.9-8.1. Record the final volume. Add sufficient aminopterin salt, preferably the disodium salt, to provide 0.4 mg/ml aminopterin (i.e. 2 mg aminopterin per 5 ml) and filter through a 0.2 micron membrane filter, desiccate by freeze drying to a solid powder, and package under sterile conditions in 10-ml vials that are sealed from the atmosphere. Resuspend with the appropriate amount of sterile water at the time of administration.

1. washthe rinse thoroughly with deionized water
2. customPrepare a first saline buffer
3. customPrepare a second saline buffer
4. additionslowly add the second saline buffer solution until the final pH is 7.9-8.1