Lactic Acid → HCID 73417092, compound

Participants

Reaction Steps

The polymer was hydrolyzed by maintaining in a 5 N aqueous sodium hydroxide solution at 60° C. for 10 hours. L-lactic acid in the resulting solution was subjected to the action of L-lactic acid hydrogenase and nicotinamide adenine dinucleotide (hereinafter simply referred to as NAD). NADH which is a reduced form of NAD generated with oxidation of lactic acid to pyruvic acid. The amount of L-lactic acid was determined by an absorbance analysis of NADH. The amount of D-lactic acid was similarly determined by action of D-lactic acid dehydrogenase and NAD. The ratio of L-lactic acid to D-lactic acid was calculated from these amounts. Additionally, it was confirmed that lactic acid did not cause racemization by maintaining L-lactic acid itself in a 5 N aqueous sodium hydroxide solution for 10 hours.

Provenance & Evidence