Lactic Acid → HCID 73417092, compound
Reaction equation
[OH-].[Na+].[C:3]([OH:8])(=[O:7])[C@H:4]([CH3:6])[OH:5].C1C=[N+]([C@@H]2O[C@H](COP(OP(OC[C@H]3O[C@@H](N4C5N=CN=C(N)C=5N=C4)[C@H](O)[C@@H]3O)(O)=O)([O-])=O)[C@@H](O)[C@H]2O)C=C(C(N)=O)C=1.C1N=C(N)C2N=CN([C@@H]3O[C@H](COP(OP(OC[C@H]4O[C@@H](N5C=C(C(N)=O)CC=C5)[C@H](O)[C@@H]4O)(O)=O)(O)=O)[C@@H](O)[C@H]3O)C=2N=1.[C:97]([OH:102])(=[O:101])[CH:98]([CH3:100])[OH:99].C(O)(=O)C(C)=O.C(O)(=O)[C@@H](C)O>>[C:3]([OH:8])(=[O:7])[C@H:4]([CH3:6])[OH:5].[C:97]([OH:102])(=[O:101])[C@@H:98]([CH3:100])[OH:99]Participants
Procedure
The polymer was hydrolyzed by maintaining in a 5 N aqueous sodium hydroxide solution at 60° C. for 10 hours. L-lactic acid in the resulting solution was subjected to the action of L-lactic acid hydrogenase and nicotinamide adenine dinucleotide (hereinafter simply referred to as NAD). NADH which is a reduced form of NAD generated with oxidation of lactic acid to pyruvic acid. The amount of L-lactic acid was determined by an absorbance analysis of NADH. The amount of D-lactic acid was similarly determined by action of D-lactic acid dehydrogenase and NAD. The ratio of L-lactic acid to D-lactic acid was calculated from these amounts. Additionally, it was confirmed that lactic acid did not cause racemization by maintaining L-lactic acid itself in a 5 N aqueous sodium hydroxide solution for 10 hours.
Sources & evidence
- ORD record
- ord-180d3c34f11c461b9c3fc68f0b6889d0
- Source dataset
- uspto-grants-1995_08
- Patent number
- US05437918
Reaction notes
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