反应详情

(6R,7R)-7-(2-Amino-4-thiazoleglyoxylamido)-3-[[[5-(hydroxycarbamoyl)-2-methyl-s-triazolo[1,5-a]pyrimidin-7-yl]thio]methyl]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid and 36.9 mg (0.121 mmol) of 1-[2-(aminooxy)-2-methylpropionyl]-2-(3,4-dihydroxybenzoyl)hydrazine hydrochloride (55 mg) (0.093 mmol) are dissolved in 7 ml of absolute dimethylacetamide. After stirring at room temperature for 20 hours a further 18.4 mg (0.060 mmol) of 1-[2-(aminooxy)-2-methylpropionyl]-2-(3,4-dihydroxybenzoyl)hydrazine hydrochloride are added. After a further 24 hours the solvent is removed at room temperature in a high vacuum and the residue is treated with water. The precipitated product is filtered off, washed well with water and dried at room temperature in a high vacuum. There are obtained 37 mg of (6R,7R)-7-[(Z)-2-(2-amino-4-thiazolyl)-2-[[1-[3-(3,4-dihydroxybenzoyl)carbazoyl]-1-methylethoxy]imino]acetamido]-3-[[[5-(hydroxycarbamoyl)-2-methyl-s-triazolo[1,5-a]pyrimidin-7-yl]thio]methyl]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid as a beige powder.

1. additionare added
2. customAfter a further 24 hours the solvent is removed at room temperature in a high vacuum
3. additionthe residue is treated with water
4. filtrationThe precipitated product is filtered off
5. washwashed well with water
6. customdried at room temperature in a high vacuum