HuagongsheHuagongshe Chemistry Print Report · For research reference only
Sodium Chloride → Phosphate Buffered Saline
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Sodium Chloride | HCID 5234 | — |
| Reactant | Ethylenediaminetetraacetic Acid | HCID 6049 | — |
| Reactant | Thioglycerol | HCID 7291 | — |
| Reactant | Sodium Azide | HCID 33557 | — |
| Reactant | Tromethamine hydrochloride | HCID 93573 | — |
| Reactant | Monobasic potassium phosphate | HCID 516951 | — |
| Catalyst | Sodium Molybdate | HCID 61424 | — |
| Solvent | Glycerol | HCID 753 | — |
| Solvent | Water | HCID 962 | — |
| Product | Phosphate Buffered Saline | HCID 24978514 | — |
Reaction Steps
(0.2 g KH2PO4, 8 g NaCl, 0.16 g Na2HPO4.7H2O in one liter of distilled water, pH 7.5). Buffer TEGT: 50 mM Tris-HCl, 10% (vol/vol) glycerol, 1 mM EDTA, 10 mM monothioglycerol and 9.92% sodium azide, pH 7.4 at 2° C. Buffer TEGT/Mo: buffer TEGT containing 19 mM sodium molybdate. Buffer TEGT/Mo/KCl: buffer TEGT/Mo containing 9.4M KCl.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US05439796 |
| Source dataset | uspto-grants-1995_08 |
| ORD ID | ord-e78d37dbf2654d9487c5fc9211aec90b |
| Source note | uspto-grants-1995_08 |