Sodium Chloride → Phosphate Buffered Saline

HRID 1755970Notes

Reaction equation

HRID 1755970 reaction equation
[OH:1][P:2]([O-:5])([OH:4])=[O:3].[K+:6].[Na+:7].[Cl-:8].C(O)C(N)(CO)CO.Cl.C(N(CC(O)=O)CC(O)=O)CN(CC(O)=O)CC(O)=O.C(O)C(O)CS.[N-]=[N+]=[N-].[Na+]>O.[O-][Mo]([O-])(=O)=O.[Na+].[Na+].OCC(CO)O>[OH:3][P:2]([O-:5])([OH:4])=[O:1].[OH:3][P:2]([O-:5])([O-:4])=[O:1].[Na+:7].[Na+:7].[Na+:7].[Cl-:8].[Cl-:8].[K+:6].[K+:6]

Participants

10

Reactant 6

HCID 5234Sodium ChlorideHCID 6049Ethylenediaminetetraacetic AcidHCID 7291ThioglycerolHCID 33557Sodium AzideHCID 93573Tromethamine hydrochlorideHCID 516951Monobasic potassium phosphate

Product 1

HCID 24978514Phosphate Buffered Saline

Reagents, catalysts & solvents 3

HCID 61424Sodium MolybdateCatalystHCID 753GlycerolSolventHCID 962WaterSolvent

Procedure

(0.2 g KH2PO4, 8 g NaCl, 0.16 g Na2HPO4.7H2O in one liter of distilled water, pH 7.5). Buffer TEGT: 50 mM Tris-HCl, 10% (vol/vol) glycerol, 1 mM EDTA, 10 mM monothioglycerol and 9.92% sodium azide, pH 7.4 at 2° C. Buffer TEGT/Mo: buffer TEGT containing 19 mM sodium molybdate. Buffer TEGT/Mo/KCl: buffer TEGT/Mo containing 9.4M KCl.

Workup

  1. customat 2° C

Sources & evidence

ORD record
ord-e78d37dbf2654d9487c5fc9211aec90b
Source dataset
uspto-grants-1995_08
Patent number
US05439796

Reaction notes

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