Ammonia → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal
Reaction equation
[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].S(O)(O)(=O)=O.[NH3:18].OP([O-])(O)=O.[K+].[NH2:25][C@H:26]([C:31]([OH:33])=[O:32])[C@H:27]([CH2:29][CH3:30])C.CC1[N+](CC2C=NC(C)=[N:45][C:46]=2[NH2:47])=CSC=1CCO.N>O>[NH2:25][C@H:26]([C:31]([OH:33])=[O:32])[CH2:27][CH2:29][CH2:30][NH:47][C:46](=[NH:45])[NH2:18].[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4]Participants
Reactant 6
Product 2
Reagents, catalysts & solvents 1
Conditions
- Temperature
- 32 °C
Procedure
The novel strain 382 and its parent strain 237 were cultivated with shaking at 32° C. for 8 hours in L-broth. Then, 60 ml of the resulted seed culture were inoculated into 1 liter jar fermenter containing 0.5 L of the a fermentation media, followed by culturing with stirring at 700 rpm at 32° C. and aeration rate 0.5 liter/min. The fermentation medium contained 100 g glucose, 9 g ammonia sulfate, 1 g KH2PO4, 0.4 g MgSO4.7H2O 0.02 g FeSO4.7H2O, 0.02 g MnSO4.7H2O, 0.2 g total nitrogen of Soybean hydrolysate, 0.3 g L-isoleucine, 0.4 mg thiamine in 1 liter of tap water (pH 7.0). During the cultivation, ammonia solution (4.7 M) was added to adjust pH to 7.0 and to supply a nitrogen source. The cultivation was carried out for 42 hours. The accumulated amount of arginine in the culture medium and the yield from glucose are shown in Table 3.
Workup
- additioncontaining 0.5 L of the a fermentation media
- stirringwith stirring at 700 rpm at 32° C.
- waitThe cultivation was carried out for 42 hours
Sources & evidence
- ORD record
- ord-fe5eebde8d224378b0e02094bb6787d2
- Source dataset
- uspto-grants-2005_01
- Patent number
- US06841365B2
Reaction notes
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