L-Aspartic Acid → L-Alanine

HRID 1761354Notes

Reaction equation

HRID 1761354 reaction equation
[NH2:1][C@H:2]([C:7]([OH:9])=[O:8])[CH2:3][C:4]([OH:6])=[O:5].O=[C:11]([CH2:15][CH2:16][C:17]([O-:19])=[O:18])[C:12]([O-:14])=[O:13]>>[NH2:1][C@H:2]([C:7]([OH:9])=[O:8])[CH2:3][C:4]([OH:6])=[O:5].[NH2:1][C@H:2]([C:7]([OH:9])=[O:8])[CH3:3].[NH2:1][C@H:11]([C:12]([OH:14])=[O:13])[CH2:15][CH2:16][C:17]([OH:19])=[O:18]

Participants

5

Reactant 2

HCID 5960L-Aspartic AcidHCID 164533Alpha-Ketoglutarate

Product 3

HCID 5950L-AlanineHCID 5960L-Aspartic AcidHCID 33032L-Glutamic Acid

Procedure

The enzymatic reaction was carried out exactly as in Example 1 except that the concentration of L-aspartic acid was 420 mM and the concentration of 2-ketoglutarate was 17 mM. Assay of the product by amino acid analysis again showed complete conversion of the L-aspartic acid to produce L-alanine and L-glutamic acid. The reaction progress of this example repeated under similar conditions but with a reaction time of 24 hrs. is illustrated graphically in FIG. 2.

Workup

  1. customThe enzymatic reaction

Sources & evidence

ORD record
ord-e7aaa977c3fa4577a51051dc505cf16c
Source dataset
uspto-grants-1989_05
Patent number
US04826766

Reaction notes

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