Nadide (JAN/USAN/INN) → Nadh

Participants

Reaction Steps

The effluent was equilibrated with 50 millimolar sodium phosphate and 1.2 millimolar NAD+, pH 7.5 at 25° C. to form the binary complex of alcohol dehydrogenase and NAD. The binary complex was then applied to the 4-[3-(N-6-aminocaproyl)aminopropyl]pyrazole dextran resin prepared as described above using 2 ml. resin per g liver and applying the solution at the rate of 10 ml./min. The affinity resin was then washed with the same buffer, 50 millimolar phosphate and 1.2 millimolar NAD pH 7.5.

Provenance & Evidence