Nadide (JAN/USAN/INN) → Nadh

HRID 1763216Notes

Reaction equation

P([O-])([O-])([O-])=O.[Na+].[Na+].[Na+].[CH:9]1[CH:14]=[N+:13]([C@@H:15]2[O:19][C@H:18]([CH2:20][O:21][P:22]([O:25][P:26]([O:29][CH2:30][C@H:31]3[O:35][C@@H:34]([N:36]4[C:40]5[N:41]=[CH:42][N:43]=[C:44]([NH2:45])[C:39]=5[N:38]=[CH:37]4)[C@H:33]([OH:46])[C@@H:32]3[OH:47])([OH:28])=[O:27])([OH:24])=[O:23])[C@@H:17]([OH:48])[C@H:16]2[OH:49])[CH:12]=[C:11]([C:50]([NH2:52])=[O:51])[CH:10]=1>>[CH:42]1[N:43]=[C:44]([NH2:45])[C:39]2[N:38]=[CH:37][N:36]([C@@H:34]3[O:35][C@H:31]([CH2:30][O:29][P:26]([O:25][P:22]([O:21][CH2:20][C@H:18]4[O:19][C@@H:15]([N:13]5[CH:12]=[C:11]([C:50]([NH2:52])=[O:51])[CH2:10][CH:9]=[CH:14]5)[C@H:16]([OH:49])[C@@H:17]4[OH:48])([OH:24])=[O:23])([OH:28])=[O:27])[C@@H:32]([OH:47])[C@H:33]3[OH:46])[C:40]=2[N:41]=1

Participants

Reactant 2

HCID 5893Nadide (JAN/USAN/INN)

HCID 24243Trisodium Phosphate

Product 1

HCID 439153Nadh

Procedure

The effluent was equilibrated with 50 millimolar sodium phosphate and 1.2 millimolar NAD+, pH 7.5 at 25° C. to form the binary complex of alcohol dehydrogenase and NAD. The binary complex was then applied to the 4-[3-(N-6-aminocaproyl)aminopropyl]pyrazole dextran resin prepared as described above using 2 ml. resin per g liver and applying the solution at the rate of 10 ml./min. The affinity resin was then washed with the same buffer, 50 millimolar phosphate and 1.2 millimolar NAD pH 7.5.

Sources & evidence

ORD record: ord-7ef2e84253db4892b819f2dcad33df97
Source dataset: uspto-grants-1978_09
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US04111751

Reaction notes

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