Phosphate → D-(-)-Fructose

HRID 1763794Notes

Reaction equation

HRID 1763794 reaction equation
[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].P([O-])([O-])([O-])=O>>[OH:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])=[O:4]

Participants

3

Reactant 2

HCID 1061PhosphateHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Product 1

HCID 5984D-(-)-Fructose

Conditions

Temperature
70 °C

Procedure

A mixture of 0.2 ml of a 1M aqueous D-glucose solution, 0.2 ml of a 0.05M aqueous MgSO4.7H2O solution, 0.2 ml of a 0.5M aqueous phosphate buffer solution (pH = 7.2) and a given amount of aqueous glucose isomerase extract is diluted with water to make 2 ml. The resulting mixture is maintained at a temperature of 70° C. for 60 minutes to effect the glucose isomerization which is terminated by the addition of 2 ml of 0.5M perchloric acid. The amount of fructose produced is determined by cystein carbazole method. The value obtained by dividing the amount of fructose produced by the amount of aqueous glucose isomerase extract is the activity, the unit of which is expressed by the abbreviation "U".

Workup

  1. extractiona given amount of aqueous glucose isomerase extract
  2. additionis diluted with water
  3. customis terminated by the addition of 2 ml of 0.5M perchloric acid

Sources & evidence

ORD record
ord-e9a25d593d69476295d6e126a3f268b5
Source dataset
uspto-grants-1978_09
Patent number
US04113568

Reaction notes

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