Phosphate → D-(-)-Fructose

HRID 1763796Notes

Reaction equation

HRID 1763796 reaction equation
[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].P([O-])([O-])([O-])=O>>[OH:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])=[O:4]

Participants

3

Reactant 2

HCID 1061PhosphateHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal2×

Product 1

HCID 5984D-(-)-Fructose

Conditions

Temperature
70 °C

Procedure

To 1l of an aqueous solution containing glucose, MgSO4.7H2O and phosphate buffer at a concentration of 0.1M, 0.005 and 0.05M, respectively, is added a given amount of insolubilized glucose isomerase and the mixture thus obtained is slowly stirred at a temperature of 70° C. for 60 minutes to effect the conversion. Then, the insolubilized isomerase is separated from the reacted solution and the amount of fructose so produced is determined according to the cystein carbazol method. The activity is calculated as in 3 above.

Workup

  1. customthe mixture thus obtained
  2. customThen, the insolubilized isomerase is separated from the reacted solution

Sources & evidence

ORD record
ord-2a1406fa3a4d424bbb183492f2017440
Source dataset
uspto-grants-1978_09
Patent number
US04113568

Reaction notes

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