Carbonate Ion → Biotin

HRID 1768513Notes

Reaction equation

HRID 1768513 reaction equation
C1C(=O)N(OC(CCSSCCC(NCCN[C:23]([CH2:25][CH2:26][CH2:27][CH2:28][C@@H:29]2[S:33][CH2:32][C@@H:31]3[NH:34][C:35]([NH:37][C@H:30]23)=[O:36])=[O:24])=O)=O)C(=O)C1.C(=O)([O-])[O-:39]>P([O-])([O-])([O-])=O>[OH:39][C:23]([CH2:25][CH2:26][CH2:27][CH2:28][C@H:29]1[C@@H:30]2[C@@H:31]([NH:34][C:35]([NH:37]2)=[O:36])[CH2:32][S:33]1)=[O:24]

Participants

4

Reactant 2

HCID 19660Carbonate IonHCID 71312116N-{2-[3-({3-[(2,5-Dioxopyrrolidin-1-yl)oxy]-3-oxopropyl}disulfanyl)propanamido]ethyl}-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide

Product 1

HCID 171548Biotin

Reagents, catalysts & solvents 1

HCID 1061PhosphateSolvent

Procedure

BSA (manufactured by Oriental Yeast K.K., F-V; 30 mg) was dissolved in 0.1M phosphate buffer (pH 7.0; 1 ml), and thereto was added an NHS-SS-biotin solution (manufactured by Pierce Chemical Co., 16.8 mg/ml in 0.1M phosphate buffer, pH 7.0; 1 ml) and the mixture was reacted at 30° C. for 1 hour. The reaction solution was centrifuged and the supernatant was subjected to Sephadex G-25 column (φ1.5 cm×30 cm, eluent: 50 mM carbonate buffer, pH 9.6) to give a biotin-BSA fraction.

Workup

  1. customthe mixture was reacted at 30° C. for 1 hour

Sources & evidence

ORD record
ord-1502fcfde30e496e922512b89924e1da
Source dataset
uspto-grants-1995_01
Patent number
US05378608

Reaction notes

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