HuagongsheHuagongshe Chemistry Print Report · For research reference only
Carbonate Ion → Biotin
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Carbonate Ion | HCID 19660 | — |
| Reactant | N-{2-[3-({3-[(2,5-Dioxopyrrolidin-1-yl)oxy]-3-oxopropyl}disulfanyl)propanamido]ethyl}-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide | HCID 71312116 | — |
| Solvent | Phosphate | HCID 1061 | — |
| Product | Biotin | HCID 171548 | — |
Reaction Steps
BSA (manufactured by Oriental Yeast K.K., F-V; 30 mg) was dissolved in 0.1M phosphate buffer (pH 7.0; 1 ml), and thereto was added an NHS-SS-biotin solution (manufactured by Pierce Chemical Co., 16.8 mg/ml in 0.1M phosphate buffer, pH 7.0; 1 ml) and the mixture was reacted at 30° C. for 1 hour. The reaction solution was centrifuged and the supernatant was subjected to Sephadex G-25 column (φ1.5 cm×30 cm, eluent: 50 mM carbonate buffer, pH 9.6) to give a biotin-BSA fraction.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US05378608 |
| Source dataset | uspto-grants-1995_01 |
| ORD ID | ord-1502fcfde30e496e922512b89924e1da |
| Source note | uspto-grants-1995_01 |