Tromethamine hydrochloride → Adenosine

HRID 1773079Notes

Reaction equation

HRID 1773079 reaction equation
CCN[C:4]([C@H:6]1[O:10][C@@H:9]([N:11]2[C:15]3[N:16]=[C:17](NCCC4C=CC(CCC(O)=O)=CC=4)[N:18]=[C:19]([NH2:20])[C:14]=3[N:13]=[CH:12]2)[C@H:8]([OH:35])[C@@H:7]1[OH:36])=[O:5].C(O)C(N)(CO)CO.Cl>CS(C)=O>[C@@H:9]1([N:11]2[C:15]3[N:16]=[CH:17][N:18]=[C:19]([NH2:20])[C:14]=3[N:13]=[CH:12]2)[O:10][C@H:6]([CH2:4][OH:5])[C@@H:7]([OH:36])[C@H:8]1[OH:35]

Participants

4

Reactant 2

HCID 93573Tromethamine hydrochlorideHCID 3086599CGS 21680

Product 1

HCID 60961Adenosine

Reagents, catalysts & solvents 1

HCID 679Dimethyl SulfoxideSolvent

Procedure

To 100 μl of the purified cell suspension, 80 μL of tritium-labeled CGS-21680 {3H-2-[p-(2-carboxyethyl)phenethylamino]-5′-(N-ethylcarbox amido)adenosine: 40 Ci/mmol; New England Nuclear [The Journal of Pharmacology and Experimental Therapeutics, Vol. 251, p. 888, 1989]} (final concentration of 6.0 mmol/L), and 20 μL of the test compound solution (dimethylsulfoxide solution of test compound diluted with Tris HCl buffer) are added. The mixture is allowed to stand at 25° C. for 120 minutes, followed by rapid suction filtration using glass-fiber filter paper (GF/C; Whatman), and then immediately washed three times with 200 μL of ice-cooled Tris HCl buffer (50 mmol/L). The glass-fiber filter paper is then placed in a vial, and MicroScinti liquid (PKI) is added. Then, the radioactivity is measured with a TopCount (PerkinElmer).

Workup

  1. additionare added
  2. filtrationfollowed by rapid suction filtration
  3. filtrationfilter paper (GF/C; Whatman)
  4. washimmediately washed three times with 200 μL of ice-
  5. temperaturecooled Tris HCl buffer (50 mmol/L)
  6. filtrationThe glass-fiber filter paper
  7. additionis added

Sources & evidence

ORD record
ord-211ecc58507c40e69e45dcb7ff357cb6
Source dataset
uspto-grants-2016_02
Patent number
US09254283B2

Reaction notes

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