反应详情

A solution of benzyl 1-oxo-2-(5-(pyridin-3-yl)-1-((2-(trimethylsilyl)ethoxy)methyl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-2,8-diazaspiro[4.5]decane-8-carboxylate (0.140 g, 0.229 mmol) in 2.00 M of Hydrogen chloride in Water (16.2 mL) was stirred at 100° C. for 18 h. The reaction was concentrated then submitted for rHPLC to give 295 (21.2 mg, 26.5% yield). ESI MS m/z=349.2 (M+1). 1H NMR (400 MHz, DMSO) δ 9.19 (d, J=1.8 Hz, 1H), 9.07 (s, 1H), 8.67 (s, 1H), 8.57 (d, J=4.6 Hz, 1H), 8.33 (d, J=8.0 Hz, 1H), 7.51 (dd, J=7.9, 4.7 Hz, 1H), 3.97 (t, J=7.0 Hz, 2H), 2.92 (d, J=12.6 Hz, 2H), 2.63 (dd, J=21.4, 9.3 Hz, 2H), 2.20 (t, J=7.0 Hz, 2H), 1.74 (td, J=12.5, 4.1 Hz, 2H), 1.48 (d, J=13.0 Hz, 2H)

1. concentrationThe reaction was concentrated