HCID 178512893, compound → 4-Nitroaniline

HRID 1781224Notes

Reaction equation

HRID 1781224 reaction equation
N1C(=O)CC[C@H]1C(NCC(N[C@H](C([NH:24][C:25]1[CH:33]=[CH:32][C:28]([N+:29]([O-:31])=[O:30])=[CH:27][CH:26]=1)=O)CCCNC(=N)N)=O)=O>C(O)(=O)C>[N+:29]([C:28]1[CH:32]=[CH:33][C:25]([NH2:24])=[CH:26][CH:27]=1)([O-:31])=[O:30]

Participants

3

Reactant 1

HCID 178512893HCID 178512893, compound

Product 1

HCID 74754-Nitroaniline

Reagents, catalysts & solvents 1

HCID 176Acetic AcidSolvent

Procedure

Next, 50 μl of the synthetic peptide substrate (2 mM), pyroGlu-Gly-Arg-p-nitroanilide (S-2444, Kabi, Sweden) were added and the mixture was incubated for 30 minutes at room temperature. The reaction was stopped by the addition of 1 ml of 10% acetic acid. The amount of p-nitroaniline produced was measured by absorbance at 405 nm. The activity was expressed as International Standard Units (IU).

Sources & evidence

ORD record
ord-e993cca47d064900bb01c95908565f69
Source dataset
uspto-grants-1997_05
Patent number
US05632986

Reaction notes

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