HCID 178512893, compound → 4-Nitroaniline

Participants

Reaction Steps

Next, 50 μl of the synthetic peptide substrate (2 mM), pyroGlu-Gly-Arg-p-nitroanilide (S-2444, Kabi, Sweden) were added and the mixture was incubated for 30 minutes at room temperature. The reaction was stopped by the addition of 1 ml of 10% acetic acid. The amount of p-nitroaniline produced was measured by absorbance at 405 nm. The activity was expressed as International Standard Units (IU).

Provenance & Evidence