tert-Butanol → HCID 10838503, compound

To a stirred solution of nucleoside 2 (1.00 g, 2.09 mmol) in THF (5.4 cm3) and water (5.4 cm3) was added sodium periodate (1.34 g, 6.27 mmol) and a 2.5% solution of osmium tetraoxide in tert-butanol (w/w, 0.265 cm3, 19 μmol). The solution was stirred at room temperature for 45 min. Water (25 cm3) was added and the solution was extracted with dichloromethane (2×50 cm3). The organic phase was washed with a saturated aqueous solution of sodium hydrogencarbonate (3×30 cm3) and dried (Na2SO4). The solvent was removed under reduced pressure and the residue was redissolved in THF (5.4 cm3) and water (5.4 cm3). The mixture was stirred at room temperature and sodium borohydride (79 mg, 2.08 mmol) was added. After stirring for 1.5 h, water (25 cm3) was added and the solution was extracted with dichloromethane (2×50 cm3). The organic phase was washed with a saturated aqueous solution of sodium hydrogencarbonate (3×30 cm3) and dried (Na2SO4). The solvent was removed under reduced pressure and the residue was purified by silica gel column chromatography using dichloromethane/methanol (98:2, v/v) as eluent to give nucleoside 9 as a white solid material (488 mg, 48%). δH (CDCl3) 9.14 (1 H, br s, NH), 7.60 (1 H, d, J 1.1, 6-H), 7.40-7.22 (10 H, m, Bn), 6.25 (1 H, d, J 7.7, 1′-H), 4.59 (1 H, d, J 7.1 Bn), 4.49 (1 H, d, J 7.1 Bn), 4.39-3.30 (m, 8H, 4′-H, 2′-H, Bn, 5′-Ha, 5-′Hb, 2″-Ha, 2″-Hb), 2.23-2.00 (2 H, m, 1″-Ha, 1″-Hb), 1.49 (3 H, d, J 0.7 CH3). δC (CDCl3) 163.5 (C-4), 151.2 (C-2), 137.1, 136.5 (Bn), 135.7 (C-6), 128.7, 128.5, 128.2, 127.8, 127.6, 127.2 (Bn), 111.3 (C-5), 87.0, 82.7, 81.1, 78.3 (C-1′, C-2′, C-3′, C-4′), 73.7, 69.6 (Bn), 64.4 (C-5′), 57.0 (C-2″), 32.4 (C-1″), 11.8 (CH3) (Found: C, 63.9; H, 6.3; N, 5.4. C26H30N2O7,0.25H 2O requires C, 64.1; H 6.3; N, 5.75%).

1. extractionthe solution was extracted with dichloromethane (2×50 cm3)
2. washThe organic phase was washed with a saturated aqueous solution of sodium hydrogencarbonate (3×30 cm3)
3. dry with materialdried (Na2SO4)
4. customThe solvent was removed under reduced pressure
5. dissolutionthe residue was redissolved in THF (5.4 cm3)
6. stirringThe mixture was stirred at room temperature
7. stirringAfter stirring for 1.5 h
8. extractionthe solution was extracted with dichloromethane (2×50 cm3)
9. washThe organic phase was washed with a saturated aqueous solution of sodium hydrogencarbonate (3×30 cm3)
10. dry with materialdried (Na2SO4)
11. customThe solvent was removed under reduced pressure
12. customthe residue was purified by silica gel column chromatography

ORD record

ord-55f8c207af1944e8a9353f9cca700cee

Source dataset

uspto-grants-2006_04

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US07034133B2