Hydrochloric Acid → HCID 178511415, compound

Ethyl (3-(N-(tert-butoxycarbonyl)amino)-1-fluoro-2-hydroxypropyl)(1,1-diethoxyethyl)phosphinate (180 mg, 4.5 mmol) was dissolved in methanol (2 mL), treated with 3 N Hydrochloric acid (20 mL, 60.0 mmol, sparged with argon immediately prior to use). The mixture was stirred at room temperature for 6 hours under an argon atmosphere. The reaction mixture was concentrated under reduced pressure, the crude product was re-dissolved in methanol (5 mL); the residual water removed by co-evaporation under reduced pressure with methanol. The crude product (70 mg) was purified by column chromatography (1×10 cm column) eluting with methylene chloride, methanol, concentrated ammonium hydroxide (6:3:1). The fractions containing product were concentrated under reduced pressure, co-evaporated with acetonitrile (2×10 ml) then with methanol (1×10 mL) and dried ovemight under high vacuum (0.1 mm Hg). This procedure afforded (3-amino-1-fluoro-2-hydroxypropyl)phosphinic acid as a white solid (40 mg, 56%). 1H NMR (300 MHz, D2O) δ 7.93 (s, 0.5H), 6.11 (s, 0.5 H), 4.60–4.20 (m, 2H), 3.42–3.08 (m, 2H).

1. customsparged with argon immediately
2. concentrationThe reaction mixture was concentrated under reduced pressure
3. dissolutionthe crude product was re-dissolved in methanol (5 mL)
4. customthe residual water removed by co-evaporation under reduced pressure with methanol
5. customThe crude product (70 mg) was purified by column chromatography (1×10 cm column)
6. washeluting with methylene chloride, methanol, concentrated ammonium hydroxide (6:3:1)
7. additionThe fractions containing product
8. concentrationwere concentrated under reduced pressure, co-evaporated with acetonitrile (2×10 ml)
9. dry with materialwith methanol (1×10 mL) and dried ovemight under high vacuum (0.1 mm Hg)

ORD record

ord-1ef84d94f06248aaa983009526fe7735

Source dataset

uspto-grants-2006_04

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US07034176B2