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Cultures of African green monkey kidney cells (Vero) cells, obtained from the American Type Culture Collection, Rockville, Md., were grown to confluence in Medium 199 supplemented with 5% fetal bovine serum, 0.075% NaHCO3, and 50 μg/ml gentamycin sulfate in 25 cm2 tissue culture flasks. Cells were infected with pseudorabies virus at a multiplicity of infection (moi) of one and incubated at room temperature for one hour to allow for virus attachment to and penetration of the cell. Under these conditions, approximately half of the cells are infected with virus. Thereafter, the cultures were rinsed three time with media and incubated in medium containing resveratrol at a final concentration of 50 μg/ml. Stock solutions of the resveratrol, obtained from Sigma Chemical Co, St. Louis, Mo. were prepared in 100% ethanol and diluted to the final concentration in tissue culture media. The maximum concentration of alcohol in the medium was 0.5%. Controls were treated identically, but were incubated without resveratrol.

1. customCultures of African green monkey kidney cells (Vero) cells, obtained from the American Type Culture Collection, Rockville, Md
2. washThereafter, the cultures were rinsed three time with media
3. additioncontaining resveratrol at a final concentration of 50 μg/ml
4. customwere prepared in 100% ethanol
5. additiondiluted to the final
6. concentrationconcentration in tissue culture media
7. concentrationThe maximum concentration of alcohol in the medium
8. additionControls were treated identically